Tuesday, 10 November 2015

Microbial Metabolism 2

Microbial Metabolism

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TCA cycle functions in _____________.
                                           
a. catabolic reactions
b. anabolic reactions
c. amphibolic reactions
d. none of these

Entner-Doudoroff pathway is found in _____________.

a. aerobic prokaryotes
b. anaerobic prokaryotes
c. both (a) and (b)
d. aerobic eukaryotes

Anoxygenic photosynthetic bacteria oxidize _____________.

a. water
b. oxgyen
c. sulfide         
d. ammonia

Which of the following(s) is/are the products of the light reactions of photosynthesis?

a. ATP only
b. NADPH only
c. ATP and O2 only
d. ATP, NADPH, and O2

Which of the following catalyze liberation of orthophosphate from organic P compounds and inorganic pyrophosphate?

a. Alkaline phosphates
b. Oxidoreductase
c. Protease
d. Hydrogenase

For each glucose molecule broken down, there are ______________ number of reduced coenzymes to be oxidized.

a. 12    
b. 8
c. 6      
d. 4

As the electron flow through the chains, much of their free energy is conserved in the form of ATP. This process is called _____________.

a. oxidative phosphorylation
b. electromotive potential
c. dehydrogenations
d. none of these


Digestive reactions where large molecules are broken down into smaller ones are referred as _____________.

a. anabolism    
b. catabolism
c. metabolism  
d. biosynthesis

Phosphate regulation has been observed in the production of _____________.

a. alkaloids
b. antibiotics
c. gibberelins
d. all of these

Most of the energy in aerobic respiration of glucose is captured by _____________.

a. substrate-level phosphorylation
b. electron transport of electrons from NADH
c. long-chain fatty acid oxidation
d. the enzyme formic-hydrogen lyase

During the reduction phase of the Calvin cycle, phosphoglyceric acid is reduced to ____________ utilizing ____________ as the reduction source.

a. phosphoglyceraldehyde; NADPH+H+
b. phosphoglyceraldehyde; NADH+H+
c. ribulose 1,5 - bisphosphate; NADH+H+
d. pyruvic acid; NADPH+H+

In order to get inorganic phosphorous into organic compounds, the phosphate ion is incorporated via _____________.

a. substrate level phosphorylation
b. oxidative phosphorylation
c. both (a) and (b)
d. DNA

The phosphate inhibition in the clavine formation with Claviceps SD58, can be counteracted by the addition of _____________.

a. alanine         
b. methionine
c. tryptophan   
d. lysine

The major route for incorporation of ammonia (NH4+) into organic compounds is via _____________.

a. reduction of pyruvate or alpha-ketoglutarate by enzymes
b. atmospheric nitrogen fixation
c. oxidation of pyruvate
d. all of these

The specific enzyme/(s) of the glyoxylate cycle is/are _____________.

a. isocitrate lyase
b. malate synthase
c. both (a) and (b)
d. anaplerotic

If ∆G of a chemical reaction has a negative value, the reaction _____________.

a. releases energy
b. requires energy
c. both (a) and (b)
d. none of these

The catabolic reaction, pentose-phosphate exists in _____________.

a. prokaryotic cells
b. eukaryotic cells
c. prokaryotic and eukaryotic cells both
d. none of these

Entner - Doudoroff pathway is not found in _____________.

a. aerobic prokaryotes
b. anaerobic prokaryotes
c. Both (a) and (b)
d. eukaryotes

Aerobic catabolism of glucose yields how much energy (ATP synthesized) relative to glucose fermentation?

a. Slightly less
b. About the same
c. Twice as much
d. More than 10 times as much

The bacteriochlorophylls used by the anoxygenic bacteria have absorbance maxima located in the ___________ portion(s) of the spectrum.

a. green           
b. blue
c. ultraviolet     
d. infrared

The relationship between an oxidation-reduction potential difference and the standard free energy change is (where n is the number of moles of electron transferred, F= Faraday's constant and E°= standard oxidation-reduction potential difference) _____________.

a. ∆G° = -nFE°
b. ∆G° = nFE°
c. ∆G° = -nFlnE°
d. ∆G° = nFlnE°

ATPase _____________.

a. synthesizes ATP, coupled to transfer of extracellular protons into the cell
b. extrudes protons from the cell coupled to the hydrolysis of ATP to ADP
c. is the enzyme that incorporates ATP into messenger RNA
d. carries out each of the reactions indicated in (a) and (b)

Which of the following is responsible for phosphate solubilization?

a. Streptococcus
b. Streptomyces
c. Bacillus
d. Clostridium

Phosphate deregulated mutants are _____________.

a. less sensitive to phosphate regulation
b. moderately sensitive to phosphate regulation
c. highly sensitive to phosphate regulation
d. none of these

The mechanism of passive or facilitated diffusion require _____________.

a. metabolic energy
b. concentration of solute against an electrochemical gradient
c. accumulation of solute against an electrochemical gradient
d. accumulation or concentration of solute against an electrochemical gradient

Nitrogen fixation is a process that requires _____________.

a. energy
b. an anaerobic environment
c. both (a) and (b)
d. an aerobic environment

Bacteriochlorophyll differs from chlorophyll in what way?

a. The chelated metal in bacteriochlorophyll is not Mg
b. There are chemical differences between the two chlorophyll in their side (R) groups
c. They have different absorption spectra
d. Both (b) and (c)

Radioisotopes are frequently used in the study of cells. Assume a culture of E. coli is grown in a culture medium containing radioactive sulphur. At the end of 48 hours, it is expected to find the radioactive label located in _____________.

a. DNA
b. enzymes
c. RNA
d. all of these

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