Tuesday, 10 November 2015

Microbial Metabolism

Microbial Metabolism

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During the carboxylation phase of the Calvin cycle, CO2 combines with _____________.

a. ribulose 1,5 - bisphosphate
b. phosphoglyceraldehyde
c. pyruvic acid
d. oxaloacetic acid

Which of the following groups contain(s) many unique coenzymes, such as coenzyme M and coenzyme F420?

a. Sulfate-reducing bacteria
b. Methanotrophs (methane-oxidizing microbes)
c. Methanogens (methane-producing microbes)
d. Acetogens (acetigens; acetate-producing microbes)

In the passive diffusion, solute molecules cross the membrane as a result of _____________.

a. concentration difference
b. pressure difference
c. ionic difference
d. all of these

In an oxygenic photosynthesis, the green and the purple bacteria do not use which of the following one as an electron source?

a. H2O
b. H2
c. H2S
d. S (elemental sulphur)

Radioisotopes are frequently used in the study of cells. Assume a culture of E. coli is grown in a culture medium containing radioactive phosphorous. At the end of 48 hours, it is expected to find the radioactive label located in _____________.

a. enzymes
b. RNA
c. phospholipids
d. all of these

Assimilatory sulfate reduction involves the nucleotide __________ during the incorporation of H2S in the production of _____________.

a. ATP; methionine
b. ATP; cytosine
c. UTP; cytosine
d. GTP; cytosine

The chlorophyll molecules used by eucaryotes and cyanobacteria absorb radiant energy in the ____________ portion(s) of the visible spectrum.

a. red
b. green
c. red and blue
d. green and ultraviolet

In aerobic respiration, the terminal electron acceptor is _____________.

a. oxygen        
b. nitrogen
c. hydrogen     
d. nitrate

Which of the following statement is correct?

a. Phosphate repression can not be eliminated by optimization of nutrient medium, deregulated medium must be used as production strains
b. Phosphate repression can be eliminated by optimization of nutrient medium, deregulated medium must be used as production strains
c. Phosphate repression can be eliminated by optimization of nutrient medium, regulated medium must be used as production strains
d. Phosphate repression can not be eliminated by optimization of nutrient medium, regulated medium must be used as production strains

The acquisition energy by glucose fermentation requires _____________.

a. substrate-level phosphorylation
b. electron transport of electrons from NADH
c. long-chain fatty acid oxidation
d. the enzyme formic-hydrogen lyase

High energy transfer compounds are capable of _____________.

a. accepting large amounts of free energy
b. transferring large amounts of free energy
c. measuring free energy
d. none of the above

Dolichol phosphate is _____________.

a. a complex lipid involved in docking vesicles with the plasma membrane
b. the anchor on which sugars assemble before transfer to proteins
c. a chaperone used in protein folding
d. a product of phospholipase C activation

The reactions of the cell that are carried out for capturing energy are called _____________.

a. catabolism
b. metabolism
c. anabolism
d. activation energy

In establishing proton gradient for chemiosmotic ATP generation by aerobic respiration the terminal electron acceptor is _____________.

a. nitrate          
b. oxygen
c. sulfate         
d. CO2

If ∆G of a chemical reaction is positive in value and keq is less than 1 then the chemical reaction will _____________.

a. proceed in reverse direction
b. proceeed in forward direction
c. not take place in any of the direction
d. none of these

The reaction, where small precursor molecules are assembled into larger organic molecules is referred as _____________.

a. anabolism
b. catabolism
c. metabolism
d. any of these

Which of the following nucleoside diphosphates is used most often in carbohydrate anabolism?

a. Uridine diphosphate
b. Adenosine diphosphate
c. Guanine diphosphate
d. Thymine diphosphate

DAHP synthetase catalyzes the condensation of _____________.

a. erythrose-4-phosphate
b. phosphoenol pyruvate
c. both (a) and (b)
d. phenylalanine

Phosphate is considered to restrict the induction of _____________.

a. primary metabolites
b. secondary metabolites
c. both (a) and (b)
d. none of these

Free energy change (∆G) of a reaction is referred as the amount of energy _____________.

a. liberated during reaction
b. taken up during reaction
c. liberated or taken up during reaction
d. none of these

Which of the following does not produce oxygen as a product of photosynthesis?

a. Oak trees
b. Purple sulfur bacteria
c. Cyanobacteria
d. Phytoplankton

When acetate is the sole source of carbon for some microorganisms, the cycle which is used, is called _____________.

a. pentose phosphate pathway
b. glycolyic pathway
c. glyoxylate pathway
d. oxaloacetate pathway

Hexose monophosphate pathway is also known as _____________.

a. phosphogluconate pathway
b. oxaloacetate pathway
c. malate pathway
d. fumerate pathway

If radioactive bicarbonate was supplied to bacterial cells, which were actively synthesizing fatty acids, it is expected to find the bulk of the radioactivity in _____________.

a. ellular bicarbonate
b. the fatty acids
c. the cytoplasmic membrane
d. nucleic acids

Standard free energy change (∆G) can be expressed as _____________.

a. ∆G° = -RTlnkeq
b. ∆G° = RTlnkeq
c. ∆G° = R/Tlnkeq
d. ∆G° = -RT/lnkeq

The glyoxylate cycle is used by some microorganisms when___________ is the sole carbon source.

a. acetate
b. nitrate
c. carbon dioxide
d. all of these

Incorporation of atmospheric N2to NH4+ occurs via the process of _____________.

a. assimilatory nitrate reduction
b. transamination
c. deamination
d. nitrogen fixation

The role of bacteriophyll in an oxygenic photosynthesis is to _____________.

a. reduce ferridoxin directly
b. reduce NADP directly
c. use light energy to energize an electron
d. transfer electrons to an intermediate in the sulfide oxidation pathway

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