Cell Cultivations
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Which of the following is the necessary step
for cultivating the microorganisms?
a. Preparing a culture medium for the growth
of microorganisms
b. Sterilizing in order to eliminate all
living microorganisms in vessel
c. Inoculating the microorganisms in the
prepared medium
d. All
of the above
Suspension cultures consist of cells and cell
aggregates, growing dispersed in___________.
a. liquid
medium
b. solid nutrient medium
c. solid or liquid medium
d. none of these
Thiobacillus thiooxidans has an optimum pH of
___________.
a. 2.0-3.5
b. 0.5-6.0
c. 6.5-7.5
d. 9.0-9.5
Balanced growth is defined as ___________.
a. cultures
undergoing balanced growth while maintaining a constant chemical composition
b. balancing the growth while controlling the
pH
c. balancing the growth while controlling the
temperature
d. balancing the growth while changing the
nutrient composition
The archaebacterium Halobacterium, an extreme
halophile, and Sulfolobus, a thermoacidophile, can be cultured in the presence
of antibiotics such as streptomycin and chloramphenicol because ___________.
a.
they contain ether-linked isoprenoids in their plasma membrane
b. they lack murein
in their cell wall
c.
they contain 80S, as opposed to 70S, ribosomes
d.
all of the above
For most bacteria, the optimum pH for growth
lies between ___________.
a.
6.5-7.5
b. 3.5-4.5
c. 4.5-5.5
d. 5.5-6.5
Which of the following is an abiotic
elicitors?
a. UV irradiation
b. Osmotic shock
c. Heavy metal ions
d. All
of these
The isolation of the gonorrhoea - causing
organisms, Neisseria gonorrhoeae,from a clinical specimen is facilitated by the
use of media containing
a.
cellulose
b. certain
antibiotics
c.
succinate
d.
none of these
Semi-solid media, prepared with agar at
concentrations of 0.5% or less are useful for the cultivation of ___________.
a. Microaerophillic
bacteria
b. Lactobacilli
c. E.coli
d. all of these
The cell mass can be measured optically by
determining the amount of light scattered by a suspension of cells. The
measurements are usually at a wavelength of ___________.
a. 300-400nm
b. 400-500nm
c. 500-600nm
d. 600-700nm
Stringent anaerobes can be grown in a media
by taking special measure as ___________.
a.
boiling the media for several minutes
b.
addition of cysteine
c.
passing through oxygen-free nitrogen
d. any of these
Radical shifts in pH can be prevented by incorporating
___________.
a. a buffer
b.
an oxidizing agent
c.
a reducing agent
d.
any of these
The protoplast can be used to ___________.
a. modify genetic information
b. create plant hybrid
c. study plant viral infections
d. all
of these
Which of the following is incorrect?
a. Mammalian cells are larger and more
complex than microorganisms
b. Their
growth rate is very fast compared to microorganisms
c. Mammalian cells are fragile
d.
Most animal cells only grow when attached to surface
The
size of a single plant cell is usually within the range of ___________.
a.
10-20μm in diameter and 25-100μm long
b. 20-40μm in
diameter and 100-200μm long
c.
40-60μm in diameter and 200-300μm long
d.
60-80μm in diameter and 300-400μm long
Which of the following is incorrect?
a.
Plant cells are larger than the bacterial or fungus cells
b.
Plant cells tend to grow in clumps
c. Plant cells are
less sensitive to shear than microbial cells
d.
Plant cells are more genetically unstable than microbial cells
Anchorage -dependent cells require ___________.
a. wettable surface
for growth
b.
dry surface for growth
c.
either (a) or (b)
d.
nothing to do with the surface
Which of the following is not an indirect
method for the measurement of cell mass?
a. Nutrient composition
b. Cell
dry weight
c. Viscosity
d. Heat evolution
Callus cultures are amorphous cell aggregates
arising from the unorganized growth of explants on an ___________.
a. liquid medium
b. solid nutrient medium
c.
aseptic solid nutrient medium
d. solid or liquid medium